Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

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3.6K Views

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07:14 min

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October 21st, 2021

DOI :

10.3791/63007-v

October 21st, 2021

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Madison Trujillo¹^,²^,³, Taylor McElroy¹^,²^,³, Taurean Brown¹^,²^,³, Pilar Simmons¹^,², Fabio Ntagwabira¹^,²^,³, Antiño R. Allen¹^,²^,³

¹Division of Radiation Health, University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences

Podsumowanie

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Neural Tissue

Hippocampal Tissue

Dissociation Protocol

Single cell Suspension

Hippocampus Isolation

Perfusion Technique

Anatomical Dissection

Tissue Viability

Rozdziały w tym wideo

0:04

Introduction

0:50

Perfusion

2:01

Dissection

3:11

Adult Brain Dissociation

4:15

Debris Removal

5:28

Live and Dead Staining

6:00

Results: Flow Cytometry Analysis Following Automated Mechanical Dissociation and Enzymatic Digestion of Mouse Brain Hippocampal Tissue

6:51

Conclusion

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