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Biology

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Nanyang Technological University

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

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2.4K Views

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06:17 min

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August 13th, 2021

DOI :

10.3791/62902-v

August 13th, 2021

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Moustafa Elkalaf¹^,², Karolína Vaněčková¹^,², Pavla Staňková¹, Zuzana Červinková¹, Jan Polák*²^,³, Otto Kučera*¹

¹Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, ²Department of Pathophysiology, Third Faculty of Medicine, Charles University, ³Department of Internal Medicine, University Hospital Kralovske Vinohrady

Podsumowanie

In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.

Przeglądaj więcej filmów

Mitochondrial Substrate Flux

Recombinant Perfringolysin O

Metformin Treatment

Seahorse XF 96 Plate

Mitochondria Metabolism

Data Acquisition

Injection Strategies

Rotenone antimycin A Mixture

Rozdziały w tym wideo

0:04

Introduction

1:03

Day 1

3:00

Day 2

4:57

Results: Mitochondrial Respiration Measurement

5:33

Conclusion

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