Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

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7.3K Views

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08:32 min

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February 14th, 2022

DOI :

10.3791/62836-v

February 14th, 2022

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Chi-Ju Kim*¹^,², Morgan D. Kuczler*¹, Liang Dong¹^,³, Junyoung Kim²^,⁴, Sarah R. Amend¹, Yoon-Kyoung Cho²^,⁴, Kenneth J. Pienta¹

¹The Brady Urological Institute, Johns Hopkins University School of Medicine, ²Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), ³Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS)

Podsumowanie

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Extracellular Vesicles

Intercellular Communication

Immunofluorescent Labeling

PC3 Cells

Cell Culture Media

Fluorescently labeled EVs

Rozdziały w tym wideo

0:04

Introduction

1:15

Extracellular Vesicle (EV) Isolation and On-Chip Immuno-Fluorescent Labeling

2:54

Incubation of the Cells with Fluorescently Labeled EVs for the EV-Uptake Assay

3:45

Confocal Microscopy and Image Processing

6:28

Results: EV Uptake Assay Using 3D Fluorescence Imaging via Confocal Microscopy

7:31

Conclusion

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