JoVE Journal

Biology

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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

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1.8K Views

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08:17 min

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August 16th, 2021

DOI :

10.3791/62790-v

August 16th, 2021

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Gianmarco Ferri¹, Fabio Azzarello¹, Francesca D'Autilia², Francesco Cardarelli¹

¹NEST Laboratory, Scuola Normale Superiore, ²Center for Nanotechnology Innovation@NEST CNI@NEST

Podsumowanie

Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties.

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Spatiotemporal Fluctuation Spectroscopy

Structural Properties

Dynamic Properties

Subcellular Nanostructures

Imaging The Right Mean Square Displacement

Fluorescent Labeling

Cellular Dynamics

Pathological Conditions

Neuro degenerative Disorders

Fluorescence Emission

Microscopy Techniques

Rozdziały w tym wideo

0:04

Introduction

0:58

Sample Preparation

2:35

Data Acquisition

3:52

iMSD Calculation

5:54

Results: Structural and Dynamic Properties of Lysosomes and Macropinosomes

7:45

Conclusion

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