An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

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4.7K Views

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08:23 min

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December 25th, 2021

DOI :

10.3791/62623-v

December 25th, 2021

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Shankari Nair¹, Samantha Cairncross², Xanthene Miles¹, Monique Engelbrecht³, Peter du Plessis¹, Julie Bolcaen¹, Randall Fisher¹, Roya Ndimba¹, Charnay Cunningham⁴, Wilner Martínez-López⁵, Christian Schunck⁶, Charlot Vandevoorde¹

¹Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, ²Department of Biotechnology, University of the Western Cape, ³Department of Medical Biosciences, University of the Western Cape, ⁴Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, ⁵Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, ⁶MetaSystems Hard & Software GmbH

Podsumowanie

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Automated Scoring Method

H2AX Foci Assay

Human Peripheral Blood Lymphocytes

Slide Scanning System

Fluorescent Microscope

Rozdziały w tym wideo

0:00

Introduction

0:44

Preparation of Samples

1:26

Sample Irradiations

1:35

Slide Preparation

2:17

Fixation and Immunofluorescence γ-H2AX Staining

3:44

Automated Metafer Scanning and Scoring

7:29

Results

8:03

Conclusion

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