Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

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6.4K Views

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14:30 min

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August 7th, 2021

DOI :

10.3791/62569-v

August 7th, 2021

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Andrea Acebes-Huerta¹, Patricia Martínez-Botía¹, Cristina Martín Martín², Ángel Bernardo¹^,³, Adrián R. Rodríguez¹, María Carmen Vicente-Ayuso⁴, Celina Benavente Cuesta⁵, Laura Gutiérrez¹^,⁶

¹Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ²Flow Cytometry and Cell Sorting Platform (ISPA), ³Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), ⁴Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), ⁵Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), ⁶Dept. of Medicine, University of Oviedo

Podsumowanie

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Immunophenotyping

Cell Sorting

Megakaryocytes MKs

Hematopoietic Progenitors

Flow Cytometry

Polyploidy

Megakaryopoiesis

Lineage specific Markers

Fluorescence activated Cell Sorting

Multi Omics Studies

Platelet Production

Human Primary Sources

Rozdziały w tym wideo

0:00

Introduction

1:17

Background

2:12

Methods: Immunophenotyping

3:50

Results

6:58

Relevance

7:42

Methods: Cell Sorting

12:27

Results and Post-Sort Analysis

12:53

Limitations and Perspective

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