JoVE Journal

Behavior

This content is Open Access.

A Within-Subject Experimental Design using an Object Location Task in Rats

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4.4K Views

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09:28 min

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May 6th, 2021

DOI :

10.3791/62458-v

May 6th, 2021

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Gülberk Bayraktar*¹^,², Kristoffer Højgaard*¹^,³, Luc Nijssen¹^,⁴, Tomonori Takeuchi¹^,⁵

¹Danish Research Institute of Translational Neuroscience (DANDRITE), Department of Biomedicine, Aarhus University, ²Universitätsklinikum Würzburg, Institut für Klinische Neurobiologie, ³Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, ⁴Faculty of Science, Radboud University, ⁵Center for Proteins in Memory - PROMEMO, Danish National Research Foundation, Department of Biomedicine, Aarhus University

Podsumowanie

This protocol provides detailed steps for an object location task with four repetitions using the same cohort of rats. Weak and strong encoding can produce short- and long-term memories. The flexibility of the protocol with repetition can be beneficial for studies involving surgical operations by saving time and labor.

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Within Subject Experimental Design

Object Location Task

Spontaneous Explorative Behavior

Automated Software

3D Spatial Cues

Geometric Shapes

Experiment Protocol

Habituation Sessions

Cleaning Procedures

Context Creation

Rozdziały w tym wideo

0:04

Introduction

0:44

Experimental Setup and Preparation of Distinct Contexts

2:02

Object Habituation

2:58

Empty Box Habituation

3:29

Context Habituation

4:24

Encoding Trial

5:25

Test Trial

6:15

Data Analysis

6:54

Results: Memory Performance After Strong and Weak Encoding

8:44

Conclusion

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