JoVE Journal
Genetics
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Nanyang Technological UniversityGenome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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09:25 min
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June 2nd, 2021
DOI :
June 2nd, 2021
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Rozdziały w tym wideo
0:04
Introduction
0:39
Preparation of Protoplasts from M. oryzae
1:48
In Vivo Crosslinking and Sonication
3:22
IP of Crosslinked Protein/DNA
4:23
Collecting and Rinsing the IP Products
5:04
Elution and Reverse Crosslinking of Protein/DNA Complexes
5:56
Purification and Recovery of DNA
6:51
DNA Repair and Solexa Library Construction
8:23
Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
8:53
Conclusion
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