A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

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2.8K Views

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09:00 min

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June 17th, 2021

DOI :

10.3791/62306-v

June 17th, 2021

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Adam D. Crawshaw¹, Emma V. Beale¹^,², Anna J. Warren¹, Andrew Stallwood¹^,³, Graham Duller¹, Jose Trincao¹, Gwyndaf Evans¹^,⁴

¹Diamond Light Source Ltd, Harwell Science and Innovation Campus, ²Paul Scherrer Institut, ³Central Laser Facility, Science and Technologies Facilities Council, Harwell Science and Innovation Campus, ⁴Rosalind Franklin Institute, Harwell Science and Innovation Campus

Podsumowanie

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Sample Preparation

Microcrystals

X ray Diffraction

Punch Freezing Robotics

CryoTEM Grids

Vitrification

Blotting Parameters

Automated Plunge Freezer

Glow Discharge

Crystallization Solution

Reservoir Solution

Blotting Time

Plunge Freezing Forceps

Carbon Film Support

Light Microscope

Rozdziały w tym wideo

0:05

Introduction

0:42

Instrument and Blotting Parameter Set-up

2:53

Crystal Harvest Process

4:01

Light Microscopy for Sample Density Assessment

4:30

Scanning Electron Microscopy for Sample Assessment

5:38

Grid Preparation for the Diffraction Experiments at VMXm

6:47

Results: Analysis of the Microcrystals on the Grids

8:24

Conclusion

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