In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

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5.4K Views

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09:48 min

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February 14th, 2021

DOI :

10.3791/62217-v

February 14th, 2021

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Hazel Hall-Roberts¹^,²^,³, Elena Di Daniel², William S. James¹, John B. Davis², Sally A. Cowley¹

¹James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, ²Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, ³UK Dementia Research Institute, Cardiff University

Podsumowanie

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In Vitro Imaging Assay

Neuroblastoma Cells

IPSC macrophages

Microglial Phagocytosis

Phenol Red free Media

Fluorescent Dye

Cell Dissociation

Centrifuge Protocol

Macrophage Media

Biological Safety Cabinet

High Content Imaging Screens

Rozdziały w tym wideo

0:04

Introduction

0:41

Preparation of Dead SH-SY5Ys

2:03

Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye

3:18

Staining of iPSC-Macrophages

4:50

Fixed-Cell High-Content Imaging

7:28

Results: Imaging, Optimization and Validation of Phagocytosis Assay

9:16

Conclusion

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