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Biology

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Nanyang Technological University

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

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3.6K Views

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10:24 min

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March 18th, 2021

DOI :

10.3791/62025-v

March 18th, 2021

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Anita Reiser¹, Daniel Woschée¹, Simon Maximilian Kempe¹, Joachim Oskar Rädler¹

¹Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany

Podsumowanie

We present a method for the acquisition of fluorescence reporter time courses from single cells using micropatterned arrays. The protocol describes the preparation of single-cell arrays, the setup and operation of live-cell scanning time-lapse microscopy and an open-source image analysis tool for automated preselection, visual control and tracking of cell-integrated fluorescence time courses per adhesion site.

Przeglądaj więcej filmów

Live cell Imaging

Single cell Arrays

Cellular Kinetics

Fluorescent Time Courses

Micro Environments

Emission Analysis

Single cell Array Fabrication

PDMs Masterpiece

Micropattern Stamps

Oxygen Plasma Treatment

Hydrophilic Surfaces

Pegylated Poly l lysine Solution

Fibronectin Solution

Homogenization Process

Rozdziały w tym wideo

0:04

Introduction

0:30

Microstructured Single Cell Array Fabrication

3:16

Cell Seeding

4:21

Perfusion System

5:13

Time-Lapse Microscopy

6:44

Fluorescent Marker mRNA Transfection

7:34

Image Analysis and Fluorescence Readout

8:29

Results: Representative Image Analysis and Fluorescence Readout

9:34

Conclusion

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