JoVE Journal
Bioengineering
Masz pełny dostęp do tej treści za pośrednictwem
Nanyang Technological UniversityRapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
13.2K Views
•
08:31 min
•
February 5th, 2021
DOI :
February 5th, 2021
•Rozdziały w tym wideo
0:04
Introduction
0:26
Cloning Parts into the Entry Vector (pYTK001) to Create Part
2:05
Assembling Part Plasmids into Cassette Plasmids
4:50
Assembling Cassette Plasmids into Multi-Gene Plasmids
5:55
Results: Multi-gene Plasmid Cloning in E. coli and Yeast
7:54
Conclusion
Powiązane Filmy
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
9.2K Views
CRISPR Guide RNA Cloning for Mammalian Systems
69.9K Views
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
9.7K Views
CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
836 Views
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
12.3K Views
The MultiBac Protein Complex Production Platform at the EMBL
15.9K Views
Mouse Genome Engineering Using Designer Nucleases
28.7K Views
Use of In Vivo Assembly for High-efficiency Plasmid Construction
321 Views
Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
37.0K Views
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
17.9K Views
ISSN 2578-2614
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone
Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.
Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.