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Genetics

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Nanyang Technological University

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

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6.1K Views

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10:28 min

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April 3rd, 2021

DOI :

10.3791/61991-v

April 3rd, 2021

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Camila F. Almeida¹, Emma C. Frair¹, Nianyuan Huang¹, Reid Neinast², Kim L. McBride²^,³^,⁴^,⁶, Robert B. Weiss⁵, Kevin M. Flanigan¹^,⁶, Nicolas Wein¹^,⁶

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, ³The Heart Center, Nationwide Children’s Hospital, ⁴Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, ⁵Department of Human Genetics, The University of Utah School of Medicine, ⁶Department of Pediatrics, The Ohio State University

Podsumowanie

This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.

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Direct Reprogramming

Human Fibroblasts

Neuromuscular Disorders

Dermal Fibroblasts

Fibroblast Growth Medium

Differentiation Medium

Viral Transduction

Puromycin Lentivirus

Rozdziały w tym wideo

0:00

Introduction

0:36

Overview

1:23

Initiation of Demal Fibroblasts Culture

2:46

Establishment of FM Cell Lines Lentvirus-hTERT Transduction

3:57

Lentivirus-MyoD Transduction

4:54

Fibroblasts Transdifferentiation

6:11

Results

9:16

Conclusion

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