JoVE Journal

Bioengineering

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Nanyang Technological University

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

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6.0K Views

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07:58 min

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November 11th, 2020

DOI :

10.3791/61931-v

November 11th, 2020

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Yuming Liu¹, Kevin W. Eliceiri¹^,²^,³

¹Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging, University of Wisconsin-Madison, ²Departments of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, ³Morgridge Institute for Research, Madison, Wisconsin, ⁴test, test

Podsumowanie

Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.

Przeglądaj więcej filmów

Fibrillar Collagen

Extracellular Matrix

Curvelet Transform

Image Representation

Individual Fiber Extraction

Region Of Interest

Quantification Tool

Post processing Analysis

Thresholding Operation

Image Annotation

Advanced Post processing

Rozdziały w tym wideo

0:04

Introduction

0:43

CT-FIRE Fiber Extraction and Region of Interest (ROI) Analysis

2:34

Advanced CT-FIRE Post-Processing

4:09

CurveAlign Fiber Analysis: CT Mode Without Boundary

5:03

CurveAlign Fiber Analysis: CT-FIRE Mode with Boundary

6:14

CurveAlign Region of Interest (ROI) Analysis

6:56

Results: Representative Tumor-Associated Human Breast Cancer Tissue Microarray (TMA) Collagen Signatures

7:26

Conclusion

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