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Nanyang Technological University

Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

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3.4K Views

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07:25 min

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September 22nd, 2020

DOI :

10.3791/61503-v

September 22nd, 2020

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Juan Antonio Suárez-Cuenca¹, Eduardo Vera-Gómez¹, Alejandro Hernández-Patricio¹, Atzín Suá Ruíz-Hernández¹, Juan Ariel Gutiérrez-Buendía¹, Carlos Ramiro Zamora-Alemán¹, Alberto Melchor-López¹, Yasser Alberto Rizo-García², Oscar Antonio Lomán-Zúñiga², Ignacio Escotto-Sánchez², Juan Miguel Rodríguez-Trejo², Rebeca Pérez-Cabeza de Vaca³, Mario Antonio Téllez-González³, Paul Mondragón-Terán³

¹Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research, Centro Médico Nacional 20 de Noviembre, ISSSTE, ²Vascular Surgery and Angiology Department, Centro Médico Nacional 20 de Noviembre, ISSSTE, ³Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research, Centro Médico Nacional 20 de Noviembre, ISSSTE

Podsumowanie

Lower limb amputation may occur even after angioplasty of obstructed vessels in Critical Limb Ischemia (CLI). Mononuclear Progenitor Cells (MPCs) reflect vascular repair. The present protocol describes the quantification of MPCs from circulation close to angioplasty, and its relationship with endothelial dysfunction and prediction of lower limb amputation.

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Amputation Prediction

Local Circulating Mononuclear Progenitor Cells

Critical Limb Ischemia

Vascular Prognosis

Endothelial Dysfunction

Risk Stratification

Vascular Surgery

Brachial Artery Diameter

Flow mediated Dilation FMD

Rutherford Classification

Fluoroscopy Guidance

Contrast Medium Injection

Rozdziały w tym wideo

0:04

Introduction

1:33

Flow-Mediated Dilation (FMD) Evaluation

2:11

Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty

4:07

Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining

5:21

MPC Quantification

6:10

Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty

7:04

Conclusion

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