Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Please note that all translations are automatically generated. Click here for the English version.

6.5K Views

•

11:37 min

•

June 18th, 2018

DOI :

10.3791/57474-v

June 18th, 2018

•

Frauke Hausburg*¹^,², Paula Müller*¹^,², Natalia Voronina*¹, Gustav Steinhoff¹^,², Robert David¹^,²

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, ²Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University

Podsumowanie

Przeglądaj więcej filmów

Keywords MicroRNA Transfer

Adult Bone Marrow derived Hematopoietic Stem Cells

Cell Engineering

Magnetic Targeting

Regenerative Medicine

Hematopoietic Stem Cell Modification

Mesenchymal Stem Cells

Bone Marrow Collection

Density Gradient Centrifugation

Mononuclear Cell Isolation

Magnetic Selection

Rozdziały w tym wideo

0:04

Title

0:53

CD133 SC Isolation

6:00

Characterization of Freshly Isolated CD133 SCs

7:21

Preparation of Transfection Complexes

8:26

CD133 SCs Transfection and Analysis

10:03

Results: Successful Engineering of miR-loaded Magnetized CD133 Cells

11:02

Conclusion

Powiązane Filmy

07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

20.5K Views

10:01

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

16.2K Views

10:03

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

9.4K Views

05:48

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

9.7K Views

08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

12.0K Views

09:43

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

9.3K Views

09:53

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

10.7K Views

09:37

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

10.9K Views

08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

4.0K Views

07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

4.5K Views