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Biology

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MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

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5.4K Views

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10:22 min

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January 16th, 2021

DOI :

10.3791/61266-v

January 16th, 2021

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Remco van Schadewijk¹, Julia R. Krug²^,³^,⁴, Andrew Webb⁵, Henk Van As²^,⁴, Aldrik H. Velders³^,⁴, Huub J. M. de Groot¹, A. Alia¹^,⁶

¹Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, ²Laboratory of Biophysics, Wageningen University & Research, ³Laboratory of BioNanoTechnology, Wageningen University & Research, ⁴MAGNEtic resonance Facility, Wageningen University & Research, ⁵C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, ⁶Institute for Medical Physics and Biophysics, Leipzig University

Podsumowanie

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

Rozdziały w tym wideo

0:00

Introduction

1:30

Microcoil Components

2:12

Sample Preparation

4:25

Mounting Sample

5:34

Determining Coil Characteristics

7:44

Representative Results

9:34

Discussion

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