Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart
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17.8K Views
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08:22 min
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April 15th, 2020
DOI :
April 15th, 2020
•1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center
Rozdziały w tym wideo
0:04
Introduction
1:11
Heart Isolation
2:34
Endocardial Endothelial Cell (EEC) Digestion
3:34
Coronary Endothelial Cell (CEC) Digestion
4:36
Endothelial Cell (EC) Sorting
6:56
Results: Representative qPCR Data CEC and EEC Isolation Verification
7:46
Conclusion
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