JoVE Journal

Immunology and Infection

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Nanyang Technological University

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

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12.7K Views

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07:47 min

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May 18th, 2020

DOI :

10.3791/61025-v

May 18th, 2020

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Theerawut Phusantisampan¹, Pattarasuda Rawiwan²^,³, Sri Rajiv Kumar Roy², Malinee Sriariyanun⁴, Win Surachetpong²^,³

¹Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), ²Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, ³Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, ⁴Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

Podsumowanie

We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.

Przeglądaj więcej filmów

Tilapia Lake Virus

Viral Detection

Segment 3 Of TiLV

Nucleic Acid Amplification

Fast Detection Method

Fish Tissue Analysis

Rozdziały w tym wideo

0:03

Introduction

0:59

RNA Extraction

2:50

Primer Design

5:20

Demonstration of RT-Lamp Assay

7:10

Results

8:19

Conclusion

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