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Developmental Biology

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Nanyang Technological University

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

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9.0K Views

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07:30 min

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December 7th, 2019

DOI :

10.3791/60550-v

December 7th, 2019

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Robert Mahen¹^,², Reiner Schulte³

¹Photonics Group, Department of Physics, Imperial College London, ²The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, University of Cambridge, ³Cambridge Institute for Medical Research, Cambridge Biomedical Campus, University of Cambridge

Podsumowanie

The purpose of this protocol is to fuse two different cell types to create hybrid cells. Fluorescence microscopy analysis of fused cells is used to track the cell of origin of cellular organelles. This assay can be used to explore how cellular structure and function respond to perturbation by cell fusion.

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Cell cell Fusion

Cellular Structure

Cellular Function

Organelle Homeostasis

Fluorescent Tagging

Cancer Cell Populations

DMEM Incubation

Mixed Cell Enrichment

Rozdziały w tym wideo

0:04

Title

0:29

Differential Fluorescent Cell Labeling

2:00

Cell-Cell Fusion

3:03

Fused Cell Enrichment

4:18

Cell-Cell Fusion Immunofluorescent Staining and Imaging

5:30

Results: Representative Fused Cell Flow Cytometry Enrichment and Imaging

6:47

Conclusion

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