Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

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5.1K Views

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07:07 min

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June 27th, 2020

DOI :

10.3791/60523-v

June 27th, 2020

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Meng Wang*¹, Yuanyuan Huang*¹, Wangpeng Gu²^,³, Haikun Wang¹

¹CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, ²School of Life Science, University of Science and Technology of China, ³State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Podsumowanie

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T Follicular Helper Cells

Germinal Center Response

Dissection Techniques

Cell Collection

Spleen Extraction

Lymph Node Analysis

DMEM Supplemented With FBS

Centrifugation

Rozdziały w tym wideo

0:04

Introduction

0:50

Influenza A/Puerto Rico/8/34 H1N1 (PR8) Virus Inoculation

1:52

Mediastinal Lymph Node (mLN) and Splenic Lymphocyte Isolation

3:22

Polyclonal T Follicular Helper (Tfh) Cell Immunostaining

4:58

Results: Representative Tfh Cell and Germinal Center Responses in a Mouse Model of Influenza A Virus Infection

6:27

Conclusion

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