Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

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9.8K Views

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09:15 min

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January 10th, 2018

DOI :

10.3791/56953-v

January 10th, 2018

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Yu-Hua Lo¹, Monica C. Pillon¹, Robin E. Stanley¹

¹Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

Podsumowanie

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Keywords X ray Crystallography

Small Angle X ray Scattering SAXS

Hybrid Structural Analysis

Affinity Chromatography

Gel Filtration

SDS PAGE

TEV Protease

Centrifugal Filtration

Rozdziały w tym wideo

0:05

Title

0:47

Nsa1 (Nop7 Associated 1) Protein Purification

3:43

Proteolytic Screening

5:14

Small-Angle X-Ray Scattering (SAXS) Data Processing, Comparison of Concentration Series, and Ensemble Modeling

7:31

Results: Modeling the Disordered C-Terminus Region of Nsa1

8:37

Conclusion

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