JoVE Journal

Chemistry

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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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11.5K Views

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08:59 min

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September 27th, 2019

DOI :

10.3791/60313-v

September 27th, 2019

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Petteri Piskunen¹, Boxuan Shen¹, Sofia Julin¹, Heini Ijäs¹^,², J. Jussi Toppari³, Mauri A. Kostiainen¹^,⁴, Veikko Linko¹^,⁴

¹Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, ²University of Jyväskylä, Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä, ³University of Jyväskylä, Nanoscience Center, Department of Physics, University of Jyväskylä, ⁴HYBER Center of Excellence, Department of Applied Physics, Aalto University

Podsumowanie

Here, we describe a protocol to create discrete and accurate inorganic nanostructures on substrates using DNA origami shapes as guiding templates. The method is demonstrated by creating plasmonic gold bowtie-shaped antennas on a transparent substrate (sapphire).

Przeglądaj więcej filmów

Substrate Nanopatterning

Inorganic Structures

Sensing Applications

Surface Enhanced Raman Spectroscopy

Optical Metasurfaces

Nanofabrication Methods

Oligonucleotides

Plasma enhanced Chemical Vapor Deposition

Amorphous Silicon

Reactive Ion Etching

Rozdziały w tym wideo

0:04

Title

1:02

DNA Origami Assembly

1:34

Substrate Preparation

2:09

Amorphous Silicon (a-Si) Layer Plasma-Enhanced Chemical Vapor Deposition (PECVD) and Oxygen Plasma Treatment

2:49

DNA Origami Deposition

3:44

Silicon Dioxide (SiO₂) Mask Growth

4:38

Reactive Ion Etching (RIE)

5:25

Physical Vapor Deposition (PVD)

6:35

Hydrofluoric Acid (HF) Liftoff

7:05

Remaining a-Si RIE

7:38

Results: Representative Bowtie DNA Origami Imaging and Functional Analyses

8:15

Conclusion

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