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Nanyang Technological University

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

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7.4K Views

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08:22 min

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February 20th, 2020

DOI :

10.3791/59442-v

February 20th, 2020

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Sarah E. Hooper¹, Amanda N. Eshelman², Ashley N. Cowan³, Alicia Roistacher⁴, Tyler S. Paneitz², Sybill K. Amelon⁵

¹Department of Veterinary Pathobiology, University of Missouri, ²College of Veterinary Medicine, University of Missouri, ³Department of Animal Science, University of Missouri, ⁴School of Natural Resources, University of Missouri, ⁵USDA USFS Northern Research Station

Podsumowanie

This article describes the deuterium oxide dilution technique in two mammals, an insectivore and carnivore, to determine total body water, lean body mass, body fat mass, and water consumption.

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Deuterium Oxide

Body Composition Assessment

Water Consumption Measurement

Non invasive Technique

Endangered Species

Wildlife Research

Subcutaneous Injections

Stock Solution Preparation

Sodium Chloride

Pharmaceutical grade Deuterium Oxide

Sterilization Procedure

Veterinary Procedure

Interfemoral Vein Puncture

Rozdziały w tym wideo

0:05

Introduction

1:07

Sterile, Isotonic, Salinated Deuterium Oxide (D₂O) Preparation

2:38

Big Brown Bat (Eptesicus fucsus) Body Composition Determination

4:34

Fourier-transform Infrared (FTIR) Spectrophotometry Analysis

6:20

Results: Representative D₂O Body Composition Assessment Validation

7:38

Conclusion

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