Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

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9.1K Views

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19:23 min

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January 16th, 2019

DOI :

10.3791/58824-v

January 16th, 2019

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Beáta Bozóki*¹^,², János András Mótyán*¹, Márió Miczi¹, Lívia Diána Gazda¹, József Tőzsér¹

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, ²Biotechnology Research Department, Gedeon Richter Plc

Podsumowanie

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Recombinant Fusion Proteins

Fluorescence Protease Assay

Protease Substrates

High throughput Screening

Maltoce Binding Protein

Rozdziały w tym wideo

0:00

Title

1:05

Generation of the Substrate-coding Expression Plasmids

2:16

Expression of the Fluorescent Substrates

3:35

Cell Disruption

4:26

Ni-NTA Magnetic Agarose Bead-based Protease Assay

12:39

PAGE Analysis

14:41

Representative Results

18:29

Conclusion

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