Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

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7.9K Views

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10:15 min

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January 7th, 2019

DOI :

10.3791/58727-v

January 7th, 2019

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Magali Court¹^,², Marie Malier¹^,², Arnaud Millet¹^,²^,³

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche, Centre Hospitalier Universitaire des Alpes

Podsumowanie

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Proteomic Analysis

Human Macrophage Polarization

Low Oxygen Environment

Macrophage Biology

Label free Quantification

Density Gradient Cell Separation

PBMCs

Centrifugation Protocol

Rozdziały w tym wideo

0:04

Title

0:40

Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)

2:34

Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)

4:32

Lysis and In-Gel Digestion

7:54

Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

9:17

Conclusion

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