A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection

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6.9K Views

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10:04 min

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January 7th, 2019

DOI :

10.3791/58523-v

January 7th, 2019

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Xiaolin Zhang¹^,², Leonie de Boer¹, Oliver W. Stockhammer¹, Dirk W. Grijpma²^,³, Herman P. Spaink⁴, Sebastian A.J. Zaat¹

¹Department of Medical Microbiology, Amsterdam UMC, ²Technical Medical Center, Department of Biomaterials Science and Technology, University of Twente, ³Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen, University of Groningen, ⁴Institute of Biology, Leiden University

Podsumowanie

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Keywords Zebrafish Embryo

In Vivo Visualization

Intravital Analysis

Biomaterial associated Infection

Staphylococcus Aureus

Bacteria only Suspension

Bacteria Microsphere Suspension

Polystyrene Microspheres

Fluorescent Staph Aureus MCherry Strain

Colony Counting

Optical Density

Rozdziały w tym wideo

0:04

Title

0:44

“Bacteria-only” and Bacteria-microspheres Suspension Preparation

3:04

Zebrafish Embryo Injection

5:50

Bacterial Culture Quantification and Infection Progression Monitoring

7:37

Results: Representative Intravital Biomaterial-associated Staphylococcus aureus Infection Analysis

9:17

Conclusion

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