Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

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18.6K Views

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06:38 min

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October 12th, 2018

DOI :

10.3791/58413-v

October 12th, 2018

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Kaya J.E. Matson¹, Anupama Sathyamurthy¹, Kory R. Johnson², Michael C. Kelly³^,⁴, Matthew W. Kelley³, Ariel J. Levine¹

¹Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, ²Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, ³Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, ⁴Single Cell Analysis Facility, Frederick National Laboratory

Podsumowanie

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Keywords Single nucleus RNA Sequencing

Spinal Cord Nuclei Isolation

Central Nervous System

Tissue Dissociation

Frozen Tissue

Cell Type specific Changes

Disease Or Injury

Rozdziały w tym wideo

0:04

Title

0:43

Preparation of the Spinal Cord

2:03

Detergent-mechanical Cell Lysis

3:25

Homogenization and Sucrose Density Gradient

5:11

Results: Representative Results of Nuclei Isolated from the Adult Mouse Spinal Cord

5:51

Conclusion

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