Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

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19.8K Views

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08:43 min

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September 18th, 2018

DOI :

10.3791/58397-v

September 18th, 2018

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Disharee Nath¹^,², Julie R. White³^,⁴, Gennady Bratslavsky¹, Leszek Kotula¹^,²

¹Department of Urology, SUNY Upstate Medical University, ²Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, ³Laboratory of Comparative Pathology, Memorial Sloan-Kettering Cancer Center, ⁴Boulder BioPATH, Inc

Podsumowanie

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Genetically engineered

Rozdziały w tym wideo

0:04

TItle

0:35

Urogenital System (UGS) Dissection

2:04

Prostate Gross Anatomy and Individual Lobe Microdissection

2:51

Tissue Processing for 3D Culture

4:41

Cell Plating and Culture and Sphere Harvest

6:38

Results: Representative Mouse Prostate Lobe Analyses

7:41

Conclusion

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