Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

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8.1K Views

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09:12 min

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July 29th, 2018

DOI :

10.3791/58054-v

July 29th, 2018

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Chenglu Xiao¹, Fang Wang², Junjie Hou¹, Xiaojun Zhu¹, Ying Luo², Jing-Wei Xiong¹

¹Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, ²Department of Biomedical Engineering, College of Engineering, Peking University

Podsumowanie

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Keywords Nanoparticle

Ventricular Resection

PAM Nanoparticle Dendrimer

ALDH1A2 SiRNA

Scrambled Negative Control SiRNA

Rozdziały w tym wideo

0:04

Title

0:37

Adult Zebrafish Ventricular Resection

2:42

Preparation of siRNA-encapsulated Nanoparticles and Injection of siRNA-encapsulated Nanoparticles into Adult Zebrafish Heart

4:29

Heart Collection, Fixation, and Evaluation of the Efficiency of siRNA Delivery

5:34

Evaluation of Nanoparticle-mediated siRNA Gene-silencing

6:38

Results: Nanoparticle-mediated siRNA Gene-silencing

8:01

Conclusion

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