A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

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13:59 min

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August 12th, 2018

DOI :

10.3791/57890-v

August 12th, 2018

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Young Yeon Kim*¹^,², Jee-Hyun Um*¹^,², Jeanho Yun¹^,²

¹Department of Biochemistry, College of Medicine, Dong-A University, ²Peripheral Neuropathy Research Center, College of Medicine, Dong-A University

Podsumowanie

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Keywords ROS

Reactive Oxygen Species

Senescence associated Secretory Phenotype

SASP

H Ras

Oncogene induced Senescence

Rozdziały w tym wideo

0:00

Title

1:10

Inducing Oncogene-induced Senescence

3:11

Monitoring Senescence by Senescence Associated ß-galactosidase (SA ß-gal) Staining

4:27

Quantifying ROS Induction during OIS

6:42

Quantifying IL-6 and IL-8 mRNA Expression for SASP Anaylsis using Real-time PCR

8:53

Quantifying the Levels of Secreted IL-6 and IL-8 Proteins for SASP Analysis using ELISA

10:56

Results: Representative ROS and SASP Expression Analysis during H-Ras-induced Senescence

12:25

Conclusion

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