Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

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7.4K Views

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09:33 min

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February 7th, 2018

DOI :

10.3791/56945-v

February 7th, 2018

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Elizabeth M. Corteselli¹, James M. Samet², Eugene A. Gibbs-Flournoy²^,³

¹Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, ²Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, ³Oak Ridge Institute for Science and Education

Podsumowanie

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Keywords Genetically encoded Fluorogenic Sensors

Toxicological Oxidative Stress

Redox Homeostasis

Confocal Microscopy

Environmental Controls

Real time Acquisition

Stage top Environmental Chamber

Numerical Aperture

Wide field Fluorescence Illumination

Rozdziały w tym wideo

0:04

Title

0:40

Microscopy Set-up

5:09

Data Acquisition

7:36

Results: Toxicological Assessment of Changes in the Intracellular E_GSH as Measured by roGFP2

8:54

Conclusion

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