Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

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9.3K Views

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10:03 min

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November 30th, 2017

DOI :

10.3791/56666-v

November 30th, 2017

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Felix M. Fuchs¹, Marina Raguse¹^,²^,³, Marcel Fiebrandt², Kazimierz Madela⁴, Peter Awakowicz², Michael Laue⁴, Katharina Stapelmann³, Ralf Moeller¹

¹Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), ²Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, ³Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, ⁴Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

Podsumowanie

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Keywords Low Pressure Plasma

Bacillus Subtilis Spores

Live Cell Microscopy

DNA Repair

Microbial Inactivation

Scanning Electron Microscopy

Fluorescence Microscopy

Spore Suspension

Aerosol Spraying

Rozdziały w tym wideo

0:05

Title

0:55

Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores

3:03

Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival

5:10

Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy

7:27

Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization

9:02

Conclusion

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