Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System

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10.9K Views

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10:55 min

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January 8th, 2018

DOI :

10.3791/56589-v

January 8th, 2018

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Nancy Adriana Espinoza-Sánchez*¹^,², Gloria Karina Chimal-Ramírez*¹, Ezequiel Moisés Fuentes-Pananá¹

¹Unidad de Investigación en Virología y Cáncer, Hospital Infantil de México Federico Gómez, ²Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México

Podsumowanie

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Keywords 3D Co culture

Monocyte breast Cancer Cell Communication

Tumor Microenvironment

Extracellular Matrix Extract ECME

U937 Cells

THP 1 Cells

Primary Breast Cancer Cells

Cell Labeling

Fluorescent Dyes

Trypsinization

Rozdziały w tym wideo

0:05

Title

1:01

Establishing 3D Co-cultures with Indirect Interaction

2:13

3D Co-cultures with Direct Interaction

6:14

Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure

8:28

Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System

10:01

Conclusion

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