Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

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6.7K Views

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12:30 min

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October 6th, 2017

DOI :

10.3791/56121-v

October 6th, 2017

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Pei-Wen Liu¹, Jia-Bao Xu¹, Yun-Qiao Dong², Xiao-Guang Chen¹, Jin-Bao Gu¹

¹Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, ²Reproductive Medical Centre of Guangdong Women and Children's Hospital

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Keywords Recombinant Mosquito Densovirus

Transduction Efficiency

Rozdziały w tym wideo

0:05

Title

1:27

Designing an Artificial Intronic Small RNA Expression Cassette

4:22

Generating a rAaeDV Construct by Cloning a miRNA or shRNA Expression Cassette into a Plasmid Backbone

5:19

Transfecting C6/36 Cells with rAaeDV Plasmids

7:33

Harvesting rAaeDV Virions from Transfected C6/36 Cells

8:53

Mosquito Transduction

10:10

Results: Overexpression and Knockdown Efficiency of rAaeDV Vectors

11:14

Conclusion

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