Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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13:21 min
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June 16th, 2017
DOI :
June 16th, 2017
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Rozdziały w tym wideo
0:05
Title
11:26
Conclusion
10:09
Results: Identification of Differentially Methylated CpG Rich Regions in Chronic Lymphocytic Leukemia (CLL) Samples
0:49
Genomic DNA Extraction and Sonication
2:06
Bead Preparation Prior to Binding with Methyl-CpG-binding Domain (MBD)-biotin Protein
3:35
Binding the MBD-biotin Protein to the Washed Beads
6:22
Removing the Unbound DNA and Eluting the Methylated DNA from the Beads
8:10
Ethanol Precipitation and the Enrichment of Methylated DNA
4:59
Binding MBD-biotin Beads with Fragmented Genomic DNA
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