Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

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12.6K Views

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12:06 min

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November 25th, 2017

DOI :

10.3791/55746-v

November 25th, 2017

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Paul R. Wratil¹^,², Rüdiger Horstkorte³

¹Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät, LMU München, ²Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, ³Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg

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Keywords Metabolic Glycoengineering

Sialic Acid

N acyl modified Mannosamoines

Polysialic Acid

Glycobiology

Receptor ligand Interactions

N acetylmannosamine

N propionylmannosamine

N butanoylmannosamine

Kelly Neuroblastoma Cells

Rozdziały w tym wideo

0:05

Title

2:26

Cell Culture and Cell Lysis for HPLC Analysis

4:28

Separation of the Membrane Fraction and Acid Hydrolysis

5:27

Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis

6:55

Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells

1:02

Synthesis of ManNProp and Related N-acyl-modefied Mannosamines

9:00

Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines

10:40

Conclusion

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