JoVE Journal

Medicine

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Nanyang Technological University

Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

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12.4K Views

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08:05 min

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July 3rd, 2017

DOI :

10.3791/55495-v

July 3rd, 2017

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Rupesh Agrawal*¹^,²^,³, Praveen Kumar Balne*², Sai Bo Bo Tun², Yeo Sia Wey², Neha Khandelwal¹, Veluchamy A. Barathi²^,⁴^,⁵

¹National Healthcare Group Eye Institute, Tan Tock Seng Hospital, ²Singapore Eye Research Institute (SERI), Singapore National Eye Center, ³School of Material Science and Engineering, Nanyang Technological University, ⁴Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, ⁵Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School

Podsumowanie

Live-cell imaging of the labeled blood cells in ocular circulation can provide information about inflammation and ischemia in diabetic retinopathy and age-related macular degeneration. A protocol to label blood cells and image the labeled cells in the retinal circulation is described.

Przeglądaj więcej filmów

Retinal Circulation

Fluorescent Dye Labeling

Sodium Fluorescein

Scanning Laser Ophthalmoscope

Blood Cell Flow Dynamics

Retinal Diseases

Rozdziały w tym wideo

0:05

Title

0:54

Isolation and Labeling of Erythrocytes (ICG) and Leukocytes (Sodium Fluorescein)

7:38

Conclusion

6:56

Results: Visualizaing the Labeled Erythrocytes and Leukocytes

3:25

Live-cell Imaging of Labeled Erythrocytes

5:26

Image Analysis Using MtrackJ Software

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