The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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8.9K Views

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08:06 min

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February 1st, 2018

DOI :

10.3791/55414-v

February 1st, 2018

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Marylène Vandevenne*¹, Mathieu Dondelinger*¹, Sami Yunus², Astrid Freischels¹, Régine Freischels¹, Oscar Crasson¹, Noureddine Rhazi¹, Pierre Bogaerts³, Moreno Galleni¹, Patrice Filée⁴

¹Life Science Department, University of Liège, ²Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, ³Laboratory of Clinical Microbiology, Catholic University of Louvain, ⁴Biotechnology Department, CER Group

Podsumowanie

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Keywords Beta lactamase

Conductimetric Biosensor

Biomolecular Interactions

Antibody antigen Interactions

Chimeric Beta lactamase

Proton Release

Immunosensors

Hybrid Protein

Protein Sample Preparation

Electrode Preparation

Electrode Regeneration

Hen egg white Lysozyme

Rozdziały w tym wideo

0:05

Title

0:52

Sensor Preparation and Regeneration

4:45

Results: Measurement of Conducticity Cganges Upon Antibiotic Hydrolysis by the Immobilization Chimeric β-Lactamase

6:35

Conclusion

2:04

Binding Assay Performed on the Sensor

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