Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

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9.2K Views

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09:34 min

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September 7th, 2017

DOI :

10.3791/55381-v

September 7th, 2017

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Jian Li¹, Holger Jahr²^,³, Wei Zheng⁴, Pei-Gen Ren¹

¹Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, ²Department of Orthopedic Surgery, Maastricht UMC+, ³Department of Orthopaedic Surgery, University Hospital RWTH, ⁴Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences

Podsumowanie

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Keywords Angiogenesis

Multiphoton Microscopy

In Vivo

3D PLGA nHAp Scaffold

Calvarial Critical Bone Defect Repair

Blood Vessel Formation

CERES

Extracellular Matrix

Lentiviral Particles

Green Fluorescent Protein

HEK293T Cells

Transduction

Rozdziały w tym wideo

0:05

Title

0:58

Scaffold Preparation

7:00

Results: 3D Scaffolds Promote Angiogenesis in Bone Defects

8:33

Conclusion

3:51

Crtitical Bone Defect Model and Scaffold Transplantation

5:21

In Vivo Imaging of Angiogenesis within the Bone Defect

2:30

Lentivirus Particle Release and Transduction Capacity

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