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JoVE Journal
Cancer Research
This content is Open Access.
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
Please note that all translations are automatically generated. Click here for the English version.
16.7K Views
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08:46 min
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March 7th, 2017
DOI :
March 7th, 2017
•1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
Rozdziały w tym wideo
0:05
Title
1:12
Mechanical Dissociation
3:25
Enzymatic Dissociation
4:50
Sucrose Gradient Centrifugation
5:49
ACK Red Blood Cell Lysis and Initial Culture Maintenance
6:50
Results: Images of Primary Cultures and Early-passage Cells
7:41
Conclusion
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