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JoVE Journal

Immunology and Infection

This content is Open Access.

Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens

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09:53 min

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February 6th, 2017

DOI :

10.3791/54991-v

February 6th, 2017

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1Division of Infection and Immunity and Systems Immunity Research Institute, Cardiff University, 2Department of Oncology, University Hospital of Lausanne (CHUV), 3Ludwig Insitutue for Cancer Research, Lausanne Branch, University of Lausanne

Here, we describe methods that we commonly employ in the laboratory to determine how the nature of the interaction between the T-cell receptor and tumor antigens, presented by human leukocyte antigens, governs T-cell functionality; these methods include protein production, X-ray crystallography, biophysics, and functional T-cell experiments.

Przeglądaj więcej filmów

X ray Crystallography
Biophysics
Functional Assays
T cell Receptor
Cancer Antigens
T cell Immunology
Receptor ligand Interactions
Protein Refolding
HLA
Beta 2 M
TCR Alpha beta Chains
Anion Exchange Chromatography
Structural Analysis
Biophysical Analysis
Immune Responses
Therapy
Diagnosis

Rozdziały w tym wideo

0:05

Title

1:05

T-cell Receptor (TCR) and Human Leukocyte Antigen-resticted Tumor-derived Peptide (pHLA) Refolding and Purification by Fast Protein Liquid Chromatography (FPLC)

3:43

Surface Plasmon Resonance (SPR) Equilibrium Binding and Thermodynamic Analyses

5:55

Protein Crystallization and X-ray Diffraction

7:39

Results: Molecular Analysis of gp100280-288 Recognition by CD8+ T-cells

8:59

Conclusion

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