An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

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29.5K Views

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08:40 min

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November 21st, 2016

DOI :

10.3791/54918-v

November 21st, 2016

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Javier Traba*¹, Pietro Miozzo*², Billur Akkaya³, Susan K. Pierce², Munir Akkaya²

¹Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, ²Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ³Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health

Podsumowanie

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Keywords Glycolysis

Mitochondrial Respiration

Lymphocytes

Extracellular Flux Assay

ACK Red Blood Cell Lysis Buffer

Rozdziały w tym wideo

0:05

Title

1:04

Tissue Processing

3:28

Magnetic B Cell and Naïve CD4⁺ T Cell Separation

4:43

Extracellular Flux Assay

6:09

Results: Representative Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) Analyses

7:48

Conclusion

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