Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

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9.9K Views

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10:30 min

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December 8th, 2016

DOI :

10.3791/54845-v

December 8th, 2016

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Luisa Trombi¹^,², Serena Danti²^,³, Sara Savelli⁴, Stefania Moscato¹, Delfo D'Alessandro²^,⁵, Claudio Ricci², Stefano Giannotti⁶, Mario Petrini¹

¹Dept. of Clinical and Experimental Medicine, University of Pisa, ²OtoLab, Azienda Ospedaliero-Universitaria Pisana (AOUP), ³Dept. of Civil and Industrial Engineering, University of Pisa, ⁴Immunohematology Operative Unit, Azienda Ospedaliero-Universitaria Pisana (AOUP), ⁵Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, ⁶II Orthopedic and Traumatologic Clinic, Azienda Ospedaliero-Universitaria Pisana (AOUP)

Podsumowanie

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Keywords Mesenchymal Stromal Cells

Autologous Culture

Fibrin Scaffold

Orthopedic Applications

Regenerative Medicine

Tissue Engineering

Bone Marrow Isolation

Plasma Separation

Clot Formation

Cell Isolation

Density Gradient Centrifugation

Rozdziały w tym wideo

0:05

Title

0:57

Preparation of Autologous Serum

2:20

Isolation of hMSCs from Bone Marrow

3:42

Culture of hMSCs in the Presence of Autologous Serum

5:19

Osteogenic Pre-induction and Recovery of hMSCs

6:20

Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay

7:30

Results: hMSCs/Fibrin Clot Generation and Application

9:10

Conclusion

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