Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

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9.9K Views

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13:10 min

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June 9th, 2017

DOI :

10.3791/55703-v

June 9th, 2017

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Mahnoush Bahjat¹, Timon A. Bloedjes¹, Amélie van der Veen¹, Guus de Wilde¹, Chiel Maas¹, Jeroen E. J. Guikema¹

¹Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

Podsumowanie

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Keywords DNA Damage

Protein Complexes

Proximity Ligation Assay

Suspension Cell Cultures

Rozdziały w tym wideo

0:05

Title

1:02

Treatment of Cells and Induction of DNA Damage

12:03

Conclusion

10:34

Results: The PLA Assay Detects Direct Protein-protein Interactions in Suspension Cell Cultures

3:21

Cytocentrifugation, Fixation, and Permeabilization

5:35

Blocking and Primary Antibody Incubation

7:19

PLA Probes, Ligation, and Amplification

9:04

Mounting and Imaging

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