Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

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7.2K Views

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11:08 min

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June 3rd, 2016

DOI :

10.3791/54108-v

June 3rd, 2016

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Alexander Brinkmann*¹^,², Camille Okom*¹^,², Eugen Kludt¹^,²^,⁴, Detlev Schild¹^,²^,³

¹Institute of Neurophysiology and Cellular Biophysics, Georg-August-Universität Göttingen, ²Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Georg-August-Universität Göttingen, ³DFG Excellence Cluster 171, Georg-August-Universität Göttingen, ⁴German Hearing Center Hannover

Podsumowanie

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Keywords Temperature induced Neuronal Activity

Activity Correlation Imaging

Nasal Epithelium

Olfactory Bulb Neurons

Olfactory Nerves

Olfactory System

Temperature Fluctuations

Calcium Imaging

Rozdziały w tym wideo

0:05

Title

0:55

Electroporation

2:06

Whole Mount Preparation

4:06

Bolus Loading

5:42

Odor Application and Temperature Experiments

7:08

Image Processing of the Post-synaptic Recordings Using Activity Correlation Imaging

8:50

Results: Bolus Loading and ACI Dissociate Thermosensitive and Chemosensitive Networks

10:16

Conclusion

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