JoVE Logo

Zaloguj się

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons

10.5K Views

08:11 min

June 19th, 2016

DOI :

10.3791/54096-v

June 19th, 2016


Przeglądaj więcej filmów

Stripe Assay

Rozdziały w tym wideo

0:05

Title

0:46

Preparing the Matrices

1:43

Stripe Generation and Laminin Coating for Neuron Cultures

3:59

Culturing Hippocampal Neurons fom E15.5 Mouse Embryos

6:36

Results: Hippocampal Neuron Growth over FLRT2 Coated Substrate

7:36

Conclusion

Powiązane Filmy

article

16:33

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

35.7K Views

article

11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

6.6K Views

article

11:56

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

15.4K Views

article

10:03

Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

12.0K Views

article

07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

9.8K Views

article

10:34

A High-content Assay for Monitoring AMPA Receptor Trafficking

7.6K Views

article

08:13

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

5.6K Views

article

09:40

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

6.5K Views

article

10:38

Using Live-Cell Imaging to Measure the Effects of Pathological Proteins on Axonal Transport in Primary Hippocampal Neurons

382 Views

article

01:16

Ocena atrakcyjnej lub odpychającej aktywności białek za pomocą neuronów hipokampa

29 Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone