Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

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45.6K Views

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10:53 min

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February 26th, 2018

DOI :

10.3791/53825-v

February 26th, 2018

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Jonathan T. Ting*¹, Brian R. Lee*¹, Peter Chong¹, Gilberto Soler-Llavina¹, Charles Cobbs², Christof Koch¹, Hongkui Zeng¹, Ed Lein¹

¹Cell Types Program, Allen Institute for Brain Science, ²The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute

Podsumowanie

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Keywords Brain Slices

NMDG Protective Recovery Method

Patch Clamp Electrophysiology

Neuronal Preservation

Adult Mouse Brain

Tissue Slicing

NMDG HEPES ACSF

Brain Slice Recovery Chamber

HEPES ACSF

Agarose Embedding

Tissue Preparation

Rozdziały w tym wideo

0:04

Title

1:05

Setup of the Slicing Station

3:15

Brain Dissection and Slicing

6:32

Optimized NMDG Protective Recovery Procedure

8:05

Results: Improved Neuronal Preservation and Reliability of Gigaohm Seal Formation with the Optimized NMDG Protective Recovery Method

9:38

Conclusion

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