Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy

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18.6K Views

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10:30 min

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May 24th, 2016

DOI :

10.3791/53776-v

May 24th, 2016

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Bailey Miskew Nichols*¹, Yoshitsugu Aoki*², Mutsuki Kuraoka², Joshua J.A. Lee¹, Shin'ichi Takeda², Toshifumi Yokota¹

¹Department of Medical Genetics, University of Alberta Faculty of Medicine and Dentistry, ²Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry

Podsumowanie

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Keywords Multi exon Skipping

Cocktail Antisense Oligonucleotides

Canine X linked Muscular Dystrophy

Duchenne Muscular Dystrophy

DMD

Antisense Exon Skipping Therapy

Cationic Liposome Transfection

Differentiation Medium

Morpholino Transfection

Rozdziały w tym wideo

0:05

Title

1:23

2'OMePS Transfection of Dog Myoblasts

2:59

Morpholino Transfection of Dog Myoblasts and RNA Extraction

5:18

Intramuscular Injections or Open Muscle Biopsy

6:42

Systemic Injections

7:39

Results: Multi-exon Skipping Using Antisense Oligonucleotides in the Dystrophin Gene in Dogs

9:37

Conclusion

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