JoVE Journal

Immunology and Infection

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Nanyang Technological University

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

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10.9K Views

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11:15 min

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May 21st, 2015

DOI :

10.3791/52836-v

May 21st, 2015

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Yun Sik Choi¹, Yong Cheol Kim¹, Keum Jin Baek¹, Youngnim Choi¹

¹Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University

Podsumowanie

Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.

Rozdziały w tym wideo

0:05

Title

1:35

Making the Probe

4:02

In Situ Hybridization

6:53

Applying an Antibody and Counter Stain

8:56

Results: Comparing Eubacterial and P. gingivalis-specific Probes and Using the Eubacterial Probe for General Bacterial Detection Purposes

10:32

Conclusion

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